You are running an unsupported browser, please upgrade your browser. Close

Email Subscription

Never miss the latest news and offers - subscribe now!

Never miss the latest news and offers - subscribe now!

Privacy Policy and Cookies

By browsing this site you accept cookies used to improve and personalize our services and marketing. Learn more about our Data Privacy Policy, including cookies, by clicking the Policy button.

By browsing this site you accept cookies used to improve and personalize our services and marketing. Learn more about our Data Privacy Policy, including cookies, by clicking the Policy button.

Virus Filtration

Robust filtration solutions for the removal of viruses from biotech and plasma processes

Virus filtration uses a membrane barrier to retain virus particles. It is a size-based removal method which uses a specifically designed polymeric membrane to retain virus particles on the surface and within the pores of the membrane. The critical performance parameters are typically the log reduction value (LRV) and the throughput. These parameters may be affected by many interacting factors such as viral load, protein concentration, presence of foulants, pressure, operating flux, ionic strength and stop start interruptions. Selecting and validating a virus filter appropriate for the expected range of conditions, is therefore, very important.

 

Pall Biotech has developed simple to use and robust products for different challenges. For the requirements of plasma processing, Pegasus™ SV4 and Ultipor® VF Grade DV20 filter have the best fit with the typical process design space; whereas for the demands of mAb processing, Pegasus Prime filters in combination with Pegasus Protect filters provide the best solution over a wide range of conditions. Both options are available in either cartridge format for integration into traditional stainless-steel processing lines, or capsule format, which may be integrated with pre-sterilized single-use manifolds.

 

Virus Filtration for mAb Processes

 

Pegasus Prime filters are unique virus removal filters which combine high flow and robust capacity. These filters can simplify process development and manufacturing processes around virus safety, and will deliver sustainable economy at all scales of mAb manufacturing.

 

Join the other pharmaceutical companies already using GMP scale Pegasus Prime devices.

 

“Pegasus Prime filter membrane was launched in 2016. In 2017, 5 pharmaceutical companies were using GMP scale Pegasus Prime devices (0.1m2 or larger). By December 2018, this more than tripled to 16 companies, of which Probiogen, BiosanaPharma, Mycenax and Roche have granted their permission to be named. Since launch, over 50 virus validation study results have been fed back to Pall, all with > 6 LRV for mammalian virus when spiked at > 6 logs.”

 

- Aernout Martens, Global Product Manager Virus Filtration at Pall Biotech

 

 

 

 

Advice and support for validating Pegasus Prime virus filters

 

Pall Biotech validation and virus filtration specialists can provide support and advice from process development through to commercialization, for the lifetime of your drug product.  Key questions are:

 

 

What virus spike should I use?

 

The basis for successful spike selection is:

 

  • Use the purest spike available (ultracentrifugation essential)
  • Use the most sensitive assay technique (large volume assays)
  • Spike only what is needed to measure your target log reduction value (LRV)

 

To learn more download the full guide here:

 

 

 

How do I incorporate a prefilter in my virus clearance study?

 

One of the key complications for validation of virus filters is the use of an adsorptive prefilter which cannot be validated as part of a robust size-removal virus filtration step.  The decision tree in this application note will guide you to the most appropriate test method. Download here:

 

 

Virus Filtration in Continuous Bioprocessing

 

Continuous bioprocessing is rapidly gaining momentum in mAb bioprocessing, providing potential advantages such as smaller facility footprints, lower investment costs, flexibility and process economy. It is important to note that continuous downstream processing relies on the same fundamental concepts as batch processing. When viral filters are used in a continuous processing, operating conditions are likely to be quite different to batch mode, with much lower flow, and longer processing times in continuous processing compared to batch.

 

Watch the below webinar to understand the quality by design (QbD) space for the application of viral filtration in continuous bioprocessing, learn about the assessment of the critical processing parameters between batch and continuous processing, and listen to a discussion on the possible validation approach for implementation of viral filtration in continuous bioprocessing.

 

 

webinar graphic

On-Demand Webinar: Virus Filtration in Continuous Bioprocessing

 

 

Virus Filtration for Plasma Processes

 

Pegasus SV4 virus removal filters combine high viral clearance and process cost control. They demonstrate highly-efficient clearance of both small "non-enveloped" and large viruses, and offer high retention robustness to pressure interruption (also described as pressure release, or start/stop).

 

The Pegasus SV4 filter membrane ensures a constant and stable flow rate when used with either dilute or complex/concentrated biological fluids, which significantly improves viral filtration economy in plasma fractionation processing.

Pegasus™ Prime Virus Filtration: Robust Retention after Pressure Interruptions (Stop and Start)

  • pgss-vrs-fltrtn.pdf

    Enter your details to download

    Country
    • Afghanistan
    • Albania
    • Algeria
    • American Samoa
    • Andorra
    • Angola
    • Anguilla
    • Antarctica
    • Antigua and Barbuda
    • Argentina
    • Aruba
    • Australia
    • Austria
    • Bahamas
    • Bahrain
    • Bangladesh
    • Barbados
    • Belgium
    • Belize
    • Benin
    • Bermuda
    • Bhutan
    • Bolivia
    • Botswana
    • Brazil
    • British Indian Ocean Territory
    • Brunei Darussalam
    • Bulgaria
    • Burkina Faso
    • Burundi
    • Cambodia
    • Cameroon
    • Canada
    • Cape Verde
    • Cayman Islands
    • Chad
    • Chile
    • China
    • Christmas Island
    • Cocos (Keeling) Islands
    • Colombia
    • Comoros
    • Cook Islands
    • Costa Rica
    • Croatia
    • Cuba
    • Cyprus
    • Czech Republic
    • Denmark
    • Djibouti
    • Dominica
    • Dominican Republic
    • Ecuador
    • Egypt
    • El Salvador
    • Equatorial Guinea
    • Eritrea
    • Estonia
    • Ethiopia
    • Falkland Islands
    • Faroe Islands
    • Fiji
    • Finland
    • France
    • French Guiana
    • French Polynesia
    • French Southern Territories
    • Gabon
    • Gambia
    • Germany
    • Ghana
    • Gibraltar
    • Greece
    • Greenland
    • Grenada
    • Guadeloupe
    • Guam
    • Guatemala
    • Guinea
    • Guinea-Bissau
    • Guyana
    • Haiti
    • Honduras
    • Hong Kong
    • Hungary
    • Iceland
    • India
    • Indonesia
    • Iraq
    • Ireland
    • Israel
    • Italy
    • Jamaica
    • Japan
    • Jordan
    • Kenya
    • Kiribati
    • Kuwait
    • Laos
    • Latvia
    • Lebanon
    • Lesotho
    • Liberia
    • Libya
    • Liechtenstein
    • Lithuania
    • Luxembourg
    • Macao
    • Madagascar
    • Malawi
    • Malaysia
    • Maldives
    • Mali
    • Malta
    • Marshall Islands
    • Martinique
    • Mauritania
    • Mauritius
    • Mayotte
    • Mexico
    • Monaco
    • Mongolia
    • Montserrat
    • Morocco
    • Mozambique
    • Myanmar
    • Namibia
    • Nauru
    • Nepal
    • Netherlands
    • Netherlands Antilles
    • New Caledonia
    • New Zealand
    • Nicaragua
    • Niger
    • Nigeria
    • Niue
    • Norfolk Island
    • Northern Mariana Islands
    • Norway
    • Oman
    • Pakistan
    • Palau
    • Palestinian Territory
    • Panama
    • Papua New Guinea
    • Paraguay
    • Peru
    • Philippines
    • Poland
    • Portugal
    • Puerto Rico
    • Qatar
    • Romania
    • Rwanda
    • Samoa
    • Saudi Arabia
    • Senegal
    • Seychelles
    • Sierra Leone
    • Singapore
    • Slovakia
    • Slovenia
    • Solomon Islands
    • Somalia
    • South Africa
    • Spain
    • Sri Lanka
    • St Kitts and Nevis
    • St Lucia
    • Sudan
    • Suriname
    • Swaziland
    • Sweden
    • Switzerland
    • Taiwan
    • Thailand
    • Togo
    • Tonga
    • Trinidad and Tobago
    • Tunisia
    • Turkey
    • Tuvalu
    • Uganda
    • United Arab Emirates
    • United Kingdom
    • United States
    • Uruguay
    • Vanuatu
    • Venezuela
    • Vietnam
    • Virgin Islands
    • Western Sahara
    • Yemen
    • Zambia
    • Zimbabwe
    Would you like to receive more information about Pall’s products and services, and related products and services from Pall’s related companies?

    By clicking the Submit or Download button below and proceeding I confirm that I have reviewed and agree with the Terms of Use and the Privacy Policy.

Pegasus™ Prime Virus Filtration: Robust  Retention after Pressure Interruptions  (Stop and Start)

 

 

 

Design Space for Plasma Derivative Applications with Pegasus™ SV4 Virus Removal Filters

  • pgss-dsgn-spc-2018.pdf

    Enter your details to download

    Country
    • Afghanistan
    • Albania
    • Algeria
    • American Samoa
    • Andorra
    • Angola
    • Anguilla
    • Antarctica
    • Antigua and Barbuda
    • Argentina
    • Aruba
    • Australia
    • Austria
    • Bahamas
    • Bahrain
    • Bangladesh
    • Barbados
    • Belgium
    • Belize
    • Benin
    • Bermuda
    • Bhutan
    • Bolivia
    • Botswana
    • Brazil
    • British Indian Ocean Territory
    • Brunei Darussalam
    • Bulgaria
    • Burkina Faso
    • Burundi
    • Cambodia
    • Cameroon
    • Canada
    • Cape Verde
    • Cayman Islands
    • Chad
    • Chile
    • China
    • Christmas Island
    • Cocos (Keeling) Islands
    • Colombia
    • Comoros
    • Cook Islands
    • Costa Rica
    • Croatia
    • Cuba
    • Cyprus
    • Czech Republic
    • Denmark
    • Djibouti
    • Dominica
    • Dominican Republic
    • Ecuador
    • Egypt
    • El Salvador
    • Equatorial Guinea
    • Eritrea
    • Estonia
    • Ethiopia
    • Falkland Islands
    • Faroe Islands
    • Fiji
    • Finland
    • France
    • French Guiana
    • French Polynesia
    • French Southern Territories
    • Gabon
    • Gambia
    • Germany
    • Ghana
    • Gibraltar
    • Greece
    • Greenland
    • Grenada
    • Guadeloupe
    • Guam
    • Guatemala
    • Guinea
    • Guinea-Bissau
    • Guyana
    • Haiti
    • Honduras
    • Hong Kong
    • Hungary
    • Iceland
    • India
    • Indonesia
    • Iraq
    • Ireland
    • Israel
    • Italy
    • Jamaica
    • Japan
    • Jordan
    • Kenya
    • Kiribati
    • Kuwait
    • Laos
    • Latvia
    • Lebanon
    • Lesotho
    • Liberia
    • Libya
    • Liechtenstein
    • Lithuania
    • Luxembourg
    • Macao
    • Madagascar
    • Malawi
    • Malaysia
    • Maldives
    • Mali
    • Malta
    • Marshall Islands
    • Martinique
    • Mauritania
    • Mauritius
    • Mayotte
    • Mexico
    • Monaco
    • Mongolia
    • Montserrat
    • Morocco
    • Mozambique
    • Myanmar
    • Namibia
    • Nauru
    • Nepal
    • Netherlands
    • Netherlands Antilles
    • New Caledonia
    • New Zealand
    • Nicaragua
    • Niger
    • Nigeria
    • Niue
    • Norfolk Island
    • Northern Mariana Islands
    • Norway
    • Oman
    • Pakistan
    • Palau
    • Palestinian Territory
    • Panama
    • Papua New Guinea
    • Paraguay
    • Peru
    • Philippines
    • Poland
    • Portugal
    • Puerto Rico
    • Qatar
    • Romania
    • Rwanda
    • Samoa
    • Saudi Arabia
    • Senegal
    • Seychelles
    • Sierra Leone
    • Singapore
    • Slovakia
    • Slovenia
    • Solomon Islands
    • Somalia
    • South Africa
    • Spain
    • Sri Lanka
    • St Kitts and Nevis
    • St Lucia
    • Sudan
    • Suriname
    • Swaziland
    • Sweden
    • Switzerland
    • Taiwan
    • Thailand
    • Togo
    • Tonga
    • Trinidad and Tobago
    • Tunisia
    • Turkey
    • Tuvalu
    • Uganda
    • United Arab Emirates
    • United Kingdom
    • United States
    • Uruguay
    • Vanuatu
    • Venezuela
    • Vietnam
    • Virgin Islands
    • Western Sahara
    • Yemen
    • Zambia
    • Zimbabwe
    Would you like to receive more information about Pall’s products and services, and related products and services from Pall’s related companies?

    By clicking the Submit or Download button below and proceeding I confirm that I have reviewed and agree with the Terms of Use and the Privacy Policy.

Design Space for Plasma Derivative Applications with Pegasus™ SV4 Virus Removal Filters